Inflammation and Immunity
The Keppler lab focusses on the modulation of the immune response through the actin cytoskeleton. The integrity of the actin cytoskeleton is crucial for cell motility and migration. Genetic defects affecting actin regulators can result in reduced B cell activation, limited antibody production and hence cause disease associated with immunodeficiency. On the other hand, impaired actin cytoskeleton regulation can lead to aberrant immune cell activation and autoimmunity. Our work aims at elucidating the mechanisms and signalling pathways of how the actin cytoskeleton can mediate both, immunodeficiency and autoimmunity.
In the Inflammation and Immunity lab at the TranslaTUM in Munich, we are particularly interested in the role of adaptive immune cells involved in our models of sepsis and inflammatory bowel diseases in order to better understand the interplay between tissue niches, metabolism and immune cells. To address these questions, we employ in vitro culture systems of immune cells as well as preclinical models of inflammatory bowel disease. To analyse cell-cell interactions during inflammation, we further improve immunological methods like advanced multi-colour flow cytometry and mass cytometry but also modern imaging techniques like whole organ imaging and image cytometry.